mea2100 system  (Mcs GmbH)

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Temporal characterisation and electrophysiological implications of TBI-induced serine/threonine kinase activity in mouse cortex

doi: 10.1007/s00018-025-05638-4

Figure Lengend Snippet: Pharmacologically Mimicking the Reduced ERK1/2 Kinase Activity and Protein Expression found at 24 h post-TBI has a Significant Influence on Spontaneous and Evoked Cortical Excitability. A (i) Temporal profile of ERK1/2 activity from 4 to 72 h post-TBI in the ipsilateral cortical hemisphere. (ii) Temporal profile of ERK1/2 activity from 4 to 72 h post-TBI in the contralateral cortical hemisphere. B Representative blots for cortical ERK1/2 expression from (i) 4 h, (ii) 24 h and (iii) 72 h post-TBI, with GAPDH housekeeper protein for normalisation. C Temporal profile of the fluctuating ERK1/2 protein expression relative to sham from 4 to 72 h post-TBI showing a correlation of ERK1/2 expression with its respective activity at matching timepoints. Grey bars show the standard error of mean of the sham hemispheres (n = 6–18). D . (i) Exemplary placement of the coronal ex vivo brain slice on the MEA chip with the recording electrodes correlating to cortical layers 1–4 outlined in red. (ii) Representative five-minute traces of the spontaneous spiking activity from the control group (black) and the ERK-inhibited group (pink). (iii) Analysis of the spontaneous activity via mean spiking frequency between the control group (black) and the ERK-inhibited group (pink). (iv) Overlaid representative LFPs of control (black) and ERK-inhibited (pink) slices from 200 μV lateral to the stimulation (0.5–5 V) electrode. Below, the correlating Input/Output Curve analysis from all recordings. (v) Overlaid representative LFPs of control (black) and ERK-inhibited (pink) slices from 200 μV above the stimulation (0.5–5 V) electrode in the direction of the dorsal cortical surface. Below, the correlating Input/Output Curve analysis from all recordings. (vi) Overlaid representative LFPs of control (black) and ERK-inhibited (pink) slices from 400 μV above the stimulation (0.5–5 V) electrode in the direction of the dorsal cortical surface. Below, the correlating Input/Output Curve analysis from all recordings. n = 11 (control) and n = 14 (ERKi). Statistical analysis for Western Blot was carried out using one-way ANOVA with post hoc Tukey’s multiple comparison test. Statistical analysis for mean spike frequency was carried out using unpaired t-test. Statistical analysis for input–output curves was carried out using two-way ANOVA with post hoc Šídák’s multiple comparisons test. p < 0.05

Article Snippet: Extracellular recordings of the spontaneous neuronal activity and amplitude responses from an input/output curve protocol were acquired in the acute cortical brain slices with a two- chamber MEA system (MEA2100 System, Multi Channel Systems MCS GmbH).

Techniques: Activity Assay, Expressing, Ex Vivo, Slice Preparation, Control, Western Blot, Comparison

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Temporal characterisation and electrophysiological implications of TBI-induced serine/threonine kinase activity in mouse cortex

doi: 10.1007/s00018-025-05638-4

Figure Lengend Snippet: Inhibition of PKC Activity Rescues the Increased Spontaneous Activity of the 24-h post-TBI Contralateral Cortical Network back to Sham Level. A Representative slices from the ipsilateral (top) and contralateral (bottom) hemispheres on the MEA chip, with the recording electrodes outlined by the turquoise and purple rectangle, respectively. B (i) Mean spike frequency of the sham cortical hemisphere at the beginning and end of the MEA recording duration. (ii) Mean spike frequency of the post-TBI ipsilateral hemisphere before and after the 25-min chelerythrine wash-in period. (iii) Mean spike frequency of the post-TBI contralateral hemisphere before and after the 25-min chelerythrine wash-in period. C (i) Sham, ipsilateral and contralateral hemisphere mean spike frequency before the wash-in of chelerythrine. (ii) Sham, ipsilateral and contralateral hemisphere mean spike frequency after the wash-in of chelerythrine to the post-TBI slices. (iii) Representative five-minute traces of the spontaneous spiking activity from the same electrode before (light purple) and after (dark purple) the wash-in of chelerythrine to the 24-h post-TBI contralateral hemisphere. Statistical analysis of each hemisphere group before and after chelerythrine application/25-min recording duration was carried out using paired t-test. Statistical analysis comparing the three groups before and the three groups after the chelerythrine wash-in to post-TBI slices was carried out using one-way ANOVA and post hoc Tukey multiple comparison test. p < 0.05

Article Snippet: Extracellular recordings of the spontaneous neuronal activity and amplitude responses from an input/output curve protocol were acquired in the acute cortical brain slices with a two- chamber MEA system (MEA2100 System, Multi Channel Systems MCS GmbH).

Techniques: Inhibition, Activity Assay, Comparison